Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 403, Issue 1, Pages 195-202Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-012-5791-3
Keywords
ELONA; Celiac disease; DNA detection; Cyclodextrin
Funding
- Commission of the European Communities [FP7-2008-ICT-216031]
- Generalitat de Catalunya
- Marie Curie Program [PIIF-GA 2009-237011 ECLO-BIOSENS]
- Ministerio de Ciencia e Innovacion, Spain [BIO2008-02841]
- ICREA Funding Source: Custom
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In this paper, we describe the development of an enzyme-linked oligonucleotide assay for the detection of a human leukocyte antigen allele associated with celiac disease based on cyclodextrin-modified polymeric surfaces. The surface of maleimide-pre-coated plates was modified with a layer of thiolated cyclodextrin polymer and used for the supramolecular capture of adamantane or ferrocene-modified carboxymethylcellulose polymers bearing DNA probes. The assay was optimised in terms of incubation time, temperature, and surface chemistry and applied to the highly sensitive and selective detection of HLA sequences with a limit of detection of 0.7 nM. A real sample analysed using this platform showed an excellent correlation with maleimide-activated plates using thiolated DNA probes.
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