Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 97, Issue 5, Pages 1340-1346Publisher
JOHN WILEY & SONS INC
DOI: 10.1002/bit.21360
Keywords
microfluidic; artificial liver; biomimetic; hepatocyte; bioreactor
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Primary hepatocytes represent a physiologically relevant model for drug toxicity screening. Here, we created a biologically inspired artificial liver sinusoid with a microfluidic endothelial-like barrier having mass transport properties similar to the liver acinus. This unit consisted of a cord of hepatocytes (50 x 30 x 500 mu m) fed by diffusion of nutrients across the microfluidic endothelial-like barrier from a convective transport vessel (10 nL/min). This configuration sustained rat and human hepatocytes for 7 days without an extracellular matrix (ECM) coating. Experiments with the metabolism mediated liver toxicant diclofenac showed no hepatotoxicity after 4 h and an IC50 of 334 +/- 41 mu M after 24 h.
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