Journal
ELECTROANALYSIS
Volume 19, Issue 15, Pages 1569-1574Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.200703903
Keywords
capillary electrophoresis; electrochemiluminescence; beta-Cyclodextrin; anisodamine; atropine; scopolamine
Categories
Ask authors/readers for more resources
This work developed a simple and sensitive method for simultaneous determination of three effective ingredients, atropine, scopolamine and anisodamine, in Flos daturae based on capillary electrophoresis coupled with electro-chemiluminescence detection. beta-Cyclodextrin was used as an additive to the running buffer for obtaining the absolute separation. The proposed method displayed the linear ranges from 0.2 to 100, 0.2 to 100 and 20 to 200 mu M for anisodamine, atropine and scopolamine with correlation coefficients more than 0.99, respectively. This method showed the relative standard deviations less than 4% and 6% for detection of migration time and peak height, respectively, and was suitable for the determination of these tropane alkaloids in plants and valuable in clinical and biochemical laboratories for quality control.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available