Journal
NATURE METHODS
Volume 4, Issue 8, Pages 641-643Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH1070
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Funding
- NCI NIH HHS [CA114747 ICMIC P50, R01 CA082214, CA119367 CCNE U54] Funding Source: Medline
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The use of R. reniformis luciferase ( RLuc) as a reporter gene in small- animal imaging has been hampered by its 481 nm peaked emission spectrum, as blue wavelengths are strongly attenuated in biological tissues. To overcome this, we generated variants of RLuc with bathochromic ( red) shifts of up to 66 nm ( 547 nm peak) that also had greater stability and higher light emission than native RLuc.
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