Journal
TRANSGENIC RESEARCH
Volume 16, Issue 4, Pages 415-428Publisher
SPRINGER
DOI: 10.1007/s11248-006-9045-z
Keywords
chloroplast; gene expression; microarray; plastid biotechnology; plastid transgene; T7 RNA polymerase
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Transcription of plastid transgenes by plastid-targeted T7 RNA polymerase (ptT7RNAP) during early seedling development in tobacco was associated with a pale-green leaf phenotype, depletion of plastid rRNAs and arrest of shoot development. Extensive analysis of mutant seedlings at the transcript level using DNA microarrays and RNA gel blotting revealed severe disruption of plastid rRNA accumulation at 4-days post-germination and reduced transcript accumulation for the essential gene clpP. Several nuclear genes encoding plastid proteins were differentially regulated in mutant seedlings over time. Ef-Tu was upregulated at 4-days post-germination and then subsequently downregulated, while RbcS was already downregulated at this early time point. The downregulation of nuclear genes encoding plastid proteins suggests disruption of plastid-to-nucleus signalling. In contrast, transcripts of three plastid genes showed increased accumulation in mutant seedlings. Transcripts of ndhC and ndhK accumulated at high levels possibly due to T7RNAP-mediated enhancement of transcription, while ptT7RNAP-mediated transcription through the phage T7 T phi terminator into the adjacent plastome increased the level of accD transcripts. The leakiness of the T phi terminator has implications for the use of T7RNAP-based expression systems in plastid biotechnology.
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