Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 405, Issue 7, Pages 2095-2106Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-012-6570-x
Keywords
Monoliths; Capillary liquid chromatography; Proteins; Peptides; Separation
Funding
- National Basic Research Program of China [2012CB910601]
- National Natural Science Foundation [21175131, 21190043]
- 863 Project [2012AA020202]
- Natural Science Foundation of China [2102004]
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Capillary liquid chromatography (cLC) has great potential for protein and peptide separation, with advantages of high efficiency, high resolution, low sample consumption, and high sensitivity when coupled with mass spectrometry. In recent years, monoliths have been widely used as the stationary phases for capillary columns, owing to easy preparation, high permeability, fast mass transfer, and low backpressure. This review summarizes recent advances (2007-2012) in monolithic columns for protein and peptide separation by cLC. After a brief introduction on the preparation of monolithic capillary columns, the emphasis of this review is focused on the recent application of such columns for protein and peptide separation by cLC. Furthermore, the challenges and potential hot points of monolithic capillary columns in the future are discussed.
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