Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 401, Issue 7, Pages 2283-2289Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-011-5291-x
Keywords
Barley; Near-infrared hyperspectral imaging; Pre-germination; Sorghum; Viability; Wheat
Funding
- Winter Cereal Trust (South Africa)
- Sorghum Trust (South Africa)
- National Research Foundation (NRF), South Africa [71390]
- NRF [UID 60958]
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Undesired germination of cereal grains diminishes process utility and economic return. Pre-germination, the term used to describe untimely germination, leads to reduced viability of a grain sample. Accurate and rapid identification of non-viable grain is necessary to reduce losses associated with pre-germination. Viability of barley, wheat and sorghum grains was investigated with near-infrared hyperspectral imaging. Principal component analyses applied to cleaned hyperspectral images were able to differentiate between viable and non-viable classes in principal component (PC) five for barley and sorghum and in PC6 for wheat. An OH stretching and deformation combination mode (1,920-1,940 nm) featured in the loading line plots of these PCs; this water-based vibrational mode was a major contributor to the viable/nonviable differentiation. Viable and non-viable classes for partial least squares-discriminant analysis (PLS-DA) were assigned from PC scores that correlated with incubation time. The PLS-DA predictions of the viable proportion correlated well with the viable proportion observed using the tetrazolium test. Partial least squares regression analysis could not be used as a source of contrast in the hyperspectral images due to sampling issues.
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