Journal
PHYTOCHEMISTRY
Volume 68, Issue 16-18, Pages 2330-2340Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2007.03.011
Keywords
C-13-labelling experiments; Quantitative 2D NMR; Specific C-13-enrichments; 2D-COSY; 2D ZQF-TOCSY
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We have investigated the reliability of 2D-COSY and 2D-TOCSY experiments to provide accurate measurements of C-13-enrichments in complex mixtures of C-13-labelled metabolites. This was done from both theoretical considerations and experimental investigations. The results showed that 2D-TOCSY but not 2D-COSY could provide accurate measurements of C-13-enrichments, provided efficient zero-quantum filters were applied during the mixing period. This approach extends the range of NMR methods applicable in C-13-labelling experiments and is suitable to investigating the dynamic behaviour of metabolic systems. (C) 2007 Elsevier Ltd. All rights reserved.
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