Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 401, Issue 8, Pages 2651-2656Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-011-5351-2
Keywords
Capillary electrophoresis/electrophoresis; Microfluidics; Microfabrication; Free-flow electrophoresis; Lab-on-a-chip
Funding
- European Social Fund (ESF) [080938819]
- Deutsche Forschungsgemeinschaft (DFG)
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We present a fast and versatile method to produce functional micro free-flow electrophoresis chips. Microfluidic structures were generated between two glass slides applying multistep liquid-phase lithography, omitting troublesome bonding steps or cost-intensive master structures. Utilizing a novel spacer-less approach with the photodefinable polymer polyethyleneglycol dimethacrylate (PEG-DA), microfluidic devices with hydrophilic channels of only 25 mu m in height were generated. The microfluidic chips feature ion-permeable segregation walls between the electrode channels and the separation bed and hydrophilic surfaces. The performance of the chip is demonstrated by free-flow electrophoretic separation of fluorescent xanthene dyes and fluorescently labeled amino acids.
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