Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 400, Issue 2, Pages 369-379Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-011-4700-5
Keywords
CdTe quantum dots; Luminescent probe; Capillary zone electrophoresis; Bioconjugation reactions
Funding
- Grant Agency of the Czech Republic [203/08/1680, 301/11/2055]
- Ministry of Education, Youth and Sports [LC06023]
- bilateral project [CZ-ES 2009-10/6 (2008CZ0017)]
- institute research plan [AV0Z40310501]
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A number of biologically important molecules, such as DNA, proteins, and antibodies, are routinely conjugated with fluorescent tags for high-sensitivity analyses. Here, the application of quantum dots in the place of bright and size-tunable luminophores is studied. Several selected bioconjugation reactions via zero-length cross-linkers, long-chain linkers, and oriented methods for linking of quantum dots with proteins were tested. Anti-ovalbumin, anti-proliferating cell nuclear antigen, anti-hemagglutinin, and anti-CD3 membrane protein as model antibodies and annexin V were used as high-specificity selectors. The reaction yield and efficiency of the prepared immunoluminescent probes were tested by capillary zone electrophoresis with laser-induced fluorescence detection.
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