Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 400, Issue 1, Pages 9-16Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-011-4708-x
Keywords
THC; LC-MS/MS; On-line SPE; Quantification
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A universal and robust analytical method for the determination of Delta 9-tetrahydrocannabinol (THC) and two of its metabolites Delta 9-(11-OH)-tetrahydrocannabinol (11-OH-THC) and 11-nor-Delta 9-carboxy-tetrahydrocannabinol (THC-COOH) in human whole blood was developed and validated for use in forensic toxicology. Protein precipitation, integrated solid phase extraction and on-line enrichment followed by high-performance liquid chromatography separation and detection with a triple quadrupole mass spectrometer were combined. The linear ranges used for the three cannabinoids were from 0.5 to 20 ng/mL for THC and 11-OH-THC and from 2.5 to 100 ng/mL for THC-COOH, therefore covering the requirements for forensic use. Correlation coefficients of 0.9980 or better were achieved for all three analytes. No relevant hydrolysis was observed for THC-COOH glucuronide with this procedure - in contrast to our previous GC-MS procedure, which obviously lead to an artificial increase of the THC-COOH concentration due to the hydrolysis of the glucuronide-conjugate occurring at high pH during the phase-transfer catalyzed methylation step.
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