Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 401, Issue 4, Pages 1273-1283Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-011-5197-7
Keywords
THC; Cannabinoid glucuronides; LC-MS/MS; Whole blood
Funding
- National Institute on Drug Abuse, National Institutes of Health
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The first method for quantifying cannabinoids and cannabinoid glucuronides in whole blood by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed and validated. Solid-phase extraction followed protein precipitation with acetonitrile. High-performance liquid chromatography separation was achieved in 16 min via gradient elution. Electrospray ionization was utilized for cannabinoid detection; both positive (Delta(9)-tetrahydrocannabinol [THC] and cannabinol [CBN]) and negative (11-hydroxy-THC [11-OH-THC], 11-nor-9-carboxy-THC [THCCOOH], cannabidiol [CBD], THC-glucuronide, and THCCOOH-glucuronide) polarity were employed with multiple reaction monitoring. Calibration by linear regression analysis utilized deuterium-labeled internal standards and a 1/x (2) weighting factor, yielding R (2) values > 0.997 for all analytes. Linearity ranged from 0.5 to 50 mu g/L (THC-glucuronide), 1.0-100 mu g/L (THC, 11-OH-THC, THCCOOH, CBD, and CBN), and 5.0-250 mu g/L (THCCOOH-glucuronide). Imprecision was < 10.5% CV, recovery was > 50.5%, and bias within +/- 13.1% of target for all analytes at three concentrations across the linear range. No carryover and endogenous or exogenous interferences were observed. This new analytical method should be useful for quantifying cannabinoids in whole blood and further investigating cannabinoid glucuronides as markers of recent cannabis intake.
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