Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 402, Issue 7, Pages 2249-2257Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-011-5442-0
Keywords
Tricyclic antidepressant drugs; Microextraction by packed sorbent; Solid-phase extraction; High-performance liquid chromatography; Human serum samples
Funding
- European Regional Development Fund [POIG 01.03.01-00-014/08-00]
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A method of microextraction by packed sorbent (MEPS) followed by liquid chromatography with diode array detection has been developed and optimized for the extraction of six tricyclic antidepressants (amitriptyline, nortriptyline, imipramine, desipramine, doxepin, nordoxepin) from human serum. The optimal parameters of MEPS extraction (type of sorbent, volume of sample, composition, and volume of washing and elution solutions) for these drugs in spiked samples were defined. The developed MEPS procedure was validated and then successfully applied to the analysis of serum reference material. The limit of detection (0.02-0.05 mu g/mL), intraday (2.7-8.8%) and interday (4.4-11.6%) precision (RSD), and the accuracy of the assay (94.5-108.8%) at three concentration levels-0.2, 0.5, and 0.8 mu g/mL-were estimated. The accuracy of the method was evaluated by the analysis of certified reference material. Moreover, the validated procedure was compared with the solid-phase extraction technique. Finally, microextraction by packed sorbent was assessed as a suitable tool in forensic and clinical methods for serum sample preparations.
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