Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 400, Issue 9, Pages 3005-3011Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-011-4973-8
Keywords
Imaging surface plasmon resonance; Multiplex immunoassay; Mycotoxins; Deoxynivalenol; Zearalenone
Funding
- Nano4Vitality program of the provinces Gelderland and Overijssel
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A prototype imaging surface plasmon resonance-based multiplex microimmunoassay for mycotoxins is described. A microarray of mycotoxin-protein conjugates was fabricated using a continuous flow microspotter device. A competitive inhibition immunoassay format was developed for the simultaneous detection of deoxynivalenol (DON) and zearalenone (ZEN), using a single sensor chip. Initial in-house validation showed limits of detection of 21 and 17 ng/mL for DON and 16 and 10 ng/mL for ZEN in extracts, which corresponds to 84 and 68 mu g/kg for DON and 64 and 40 mu g/kg for ZEN in maize and wheat samples, respectively. Finally, the results were critically compared with data obtained from liquid chromatography-mass spectrometry confirmatory analysis method and found to be in good agreement. The described multiplex immunoassay for the rapid screening of several mycotoxins meets European Union regulatory limits and represents a robust platform for mycotoxin analysis in food and feed samples.
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