Journal
PARASITE IMMUNOLOGY
Volume 29, Issue 8, Pages 387-394Publisher
BLACKWELL PUBLISHING
DOI: 10.1111/j.1365-3024.2007.00951.x
Keywords
allele-specific; antibodies; immunity; MSP3; Plasmodium falciparum
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Funding
- Medical Research Council [MC_U190081993] Funding Source: researchfish
- Medical Research Council [MC_U190081993] Funding Source: Medline
- Wellcome Trust Funding Source: Medline
- MRC [MC_U190081993] Funding Source: UKRI
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Many studies on the role of merozoite surface protein 3 (MSP3) in immunity against malaria have focused on a conserved section of MSP3. New evidence suggests that polymorphic sequences within MSP3 are under immune selection. We report a detailed analysis of naturally-acquired antibodies to allele-specific and conserved parts of MSP3 in a Kenyan cohort. Indirect and competition ELISA to heterologous recombinant MSP3 proteins were used for antibody assays, and parasites were genotyped for msp3 alleles. Antibody reactivity to allele-specific and conserved epitopes of MSP3 was heterogenous between individuals. Overall, the prevalence of allele-specific antibody reactivity was significantly higher (3D7-specific 54%, K1-specific 4%) than that to a recombinant protein representing a conserved portion of C-terminal MSP3 (24%, P < 0 center dot 01). The most abundant IgG subclass was IgG3, followed by IgG1. Allele-specific reactivity to the KI-type of MSP3 was associated with a lower risk of clinical malaria episodes during a 6-month follow-up in individuals who were parasitized at the start of the malaria transmission season (Relative risk 0 center dot 41 with 95% confidence interval 0 center dot 20-0 center dot 81, P = 0 center dot 011). The potential importance of allele-specific immunity to MSP3 should be considered in addition to immunity to conserved epitopes, in the development of an MSP3 malaria vaccine.
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