Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 397, Issue 2, Pages 687-693Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-010-3571-5
Keywords
Dried blood spots; Guthrie card; Metronidazole; HPLC
Ask authors/readers for more resources
A selective and sensitive high-performance liquid chromatography method with UV detection for the determination of metronidazole in dried blood spots (DBS) has been developed and validated. DBS samples [spiked or patient samples] were prepared by applying blood (30 mu L) to Guthrie cards. Discs (6 mm diameter) were punched from the cards and extracted using water containing the internal standard, tinidazole. The extracted sample was chromatographed without further treatment using a reversed phase system involving a Symmetry (R) C18 (5 mu m, 3.9x150 mm) preceded by a Symmetry (R) guard column of matching chemistry and a detection wavelength of 317 nm. The mobile phase comprised acetonitrile/0.01 M phosphate solution (KH(2)PO(4)), pH 4.7, 15: 85, v/v, with a flow rate of 1 mL/min. The calibration was linear over the range 2.5-50 mg/mL. The limits of detection and quantification were 0.6 and 1.8 mu g/mL, respectively. The method has been applied to the determination of 203 DBS samples from neonatal patients for a phamacokinetic/pharmacodynamic study.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available