Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 397, Issue 8, Pages 3173-3178Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-010-3821-6
Keywords
Transfection; Nucleic acid; Gene; Single cell
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Transfection is a powerful analytical tool enabling study of the function of genes and gene products in cells. The transfection methods are broadly classified into three groups; biological, chemical, and physical. These methods have advanced to make it possible to deliver nucleic acids to specific subcellular regions of cells by use of a precisely controlled laser-microcope system. The combination of point-directed transfection and mRNA transfection is a new way of studying the function of genes and gene products. However, each method has its own advantages and disadvantages so the optimum method depends on experimental design and objective.
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