Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 397, Issue 8, Pages 3185-3193Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-010-3723-7
Keywords
Neuropeptides; Mass spectrometric imaging; MALDI-MS; Direct tissue profiling; Single-cell measurements
Funding
- National Science Foundation [CHE-0449991]
- National Institutes of Health [1R01DK071801]
- Alfred P. Sloan Research Fellowship
- Vilas Associate Award
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Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) is a rapid and sensitive analytical method that is well suited for determining molecular weights of peptides and proteins from complex samples. MALDI-MS can be used to profile the peptides and proteins from single-cell and small tissue samples without the need for extensive sample preparation. Furthermore, the recently developed MALDI imaging technique enables mapping of the spatial distribution of signaling molecules in tissue samples. Several examples of signaling molecule analysis at the single-cell and single-organ levels using MALDI-MS technology are highlighted followed by an outlook of future directions.
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