Journal
BIOMEDICINE & PHARMACOTHERAPY
Volume 61, Issue 7, Pages 451-454Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biopha.2007.03.007
Keywords
epigenetic factors; glucocorticoids; glucocorticoid receptor
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The glucocorticoid receptor (GR) is mainly expressed as nine-exon alternatively spliced variants, encoding functional GRU and nonfunctional GRP. Overexpression of GRP splice variant was found in glucocorticoid-resistant patients with some autoimmune diseases and hernatological malignancies. Employing reverse transcription, real-time quantitative PCR, and western blot analysis, we determined an effect of trichostatin A (T SA), sodium butyrate (NaBu) and 5-aza- 2'-deoxycytidine (5-dAzaC) on GR alpha and GR beta expression in Hut-78 T- and kaji B-lymphoma cell lines. We found that TSA, NaBu, and 5-dAzaC significantly increase the expression of GR alpha transcript and protein, whereas GRP transcript and protein expression was profoundly decreased in Hut-78 T- and Raji B- lymphoma cell lines. Our observation suggests that changes of epigenetic milieu inside cells may alter the expression of GRa and GRP isoforms. (c) 2007 Elsevier Masson SAS. All rights'reserved.
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