Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 397, Issue 7, Pages 2977-2986Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-010-3859-5
Keywords
Estrogens; MIPs; SPE; HPLC-UV/EC
Funding
- Ministry of Science and Higher Education (Warsaw, Poland) [R05 037 03]
- Ceepus II, Socrates Erasmus Grant
- VEGA [1/4461/07]
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The isolation and preconcentration of estrogens from new types of biological samples (acellular and protein-free simulated body fluid) by molecularly imprinted solid-phase extraction has been described. In this technique, supramolecular receptors, namely molecularly imprinted polymers (MIPs) are used as a sorbent material. The recognition sites of MIPs were prepared by non-covalent multiple interactions and formed with the target 17 beta-estradiol as a template molecule. High-performance liquid chromatography with spectroscopic UV, selective, and a sensitive electrochemical CoulArray detector was used for the determination of 17 beta-estradiol, estrone, and estriol in simulated body fluid which mimicked human plasma.
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