Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 397, Issue 1, Pages 233-241Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-010-3517-y
Keywords
2-Nitrofluorene; 2,7-Dinitrofluorene; DNA biosensor; Screen printed carbon paste electrode; Electrochemical detection; DNA damage
Funding
- Ministry of Education, Youth and Sports of the Czech Republic [LC 06035, MSM 0021620857, RP 14/63]
- European Union
- Ministry of Education of the Slovak Republic [1/0852/08]
- Slovak Academy of Sciences
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An electrochemical DNA biosensor based on the screen printed carbon paste electrode (SPCPE) with an immobilized layer of calf thymus double-stranded DNA has been used for in vitro investigation of the interaction between genotoxic nitro derivatives of fluorene (namely 2-nitrofluorene and 2,7-dinitrofluorene) and DNA. Two types of DNA damage have been detected at the DNA/SPCPE biosensor: first, that caused by direct association of the nitrofluorenes, for which an intercalation association has been found using the known DNA intercalators [Cu(phen)(2)](2+) and [Co(phen)(3)](3+) as competing agents, and, second, that caused by short-lived radicals generated by electrochemical reduction of the nitro group (observable under specific conditions only).
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