Journal
JOURNAL OF CLINICAL VIROLOGY
Volume 39, Issue 4, Pages 312-317Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jcv.2007.05.006
Keywords
virus clearance; measles; Africa; HIV-1; RT-PCR
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Funding
- NIAID NIH HHS [AI23047] Funding Source: Medline
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Background: Measles virus (MV) RNA was detected I month after hospitalization with measles in more than half of Zambian children but the duration of detectable RNA was not determined. Objectives: To characterize the time course of MV clearance and identify factors associated with presence of viral RNA at late times after clinical recovery from infection. Study design: Blood, urine and nasopharyngeal specimens from 49 Zambian children With laboratory-confirmed measles were collected a median of 100 days (range 65-118) after rash onset. Samples were assayed for MV nucleocapsid and hemagglutinin RNA by reverse transcriptase-polymerase chain reaction. Amplified products were sequenced. Selected immunologic studies were performed. Results: MV RNA was detected in at least one specimen from 18 children (37%). Eighteen percent of 44 blood mononuclear cell, 23% of 30 nasopharyngeal and 50% of 6 urine specimens were positive. Detection was not associated with HIV- I infection, % CD4' T lymphocytes, plasma interieukin- 10 levels or persistent MV-specific IgM. The MV genotype was D2 and sequences of late specimens were the same as specimens collected during acute illness. Conclusions: Presence of viral RNA at multiple sites more than 3 months after acute disease suggests that clearance of MV-infected cells occurs over many months. (c) 2007 Elsevier B.V. All rights reserved.
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