Journal
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
Volume 12, Issue 6, Pages 913-918Publisher
SPRINGER
DOI: 10.1007/s00775-007-0244-8
Keywords
human serum albumin; copper(II); conditional stability constant
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The conditional stability constant at pH 7.4 for Cu(II) binding at the N-terminal site (NTS) of human serum albumin (HSA) was determined directly by competitive UV-vis spectroscopy titrations using nitrilotriacetic acid (NTA) as the competitor in 100 mM NaCl and 100 mM N-( 2- hydroxyethyl) piperazine-N'-ethanesulfonic acid (Hepes). The log K (c)(NTS) value of 12.0 +/- 0.1 was determined for HSA dissolved in 100 mM NaCl. A false log log K-NTS(c) value of 11.4 +/- 0.1 was obtained in the 100 mM Hepes buffer, owing to the formation of a ternary Cu(NTA)( Hepes) complex. The impact of the picomolar affinity of HSA for Cu(II) on the availability of these ions in neurodegenerative disorders is briefly discussed.
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