Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 396, Issue 4, Pages 1473-1479Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-009-3368-6
Keywords
HILIC; NanoLCFT-ICRMS; Glycan profiling; N-linked glycans; Protein depletion
Funding
- National Institutes of Health [R33 CA105295]
- W.M. Keck Foundation
- North Carolina State University
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We report the results of abundant plasma protein depletion on the analysis of underivatized N-linked glycans derived from plasma proteins by nanoLC Fourier-transform ion cyclotron resonance mass spectrometry. N-linked glycan profiles were compared between plasma samples where the six most abundant plasma proteins were depleted (n = 3) through a solid-phase immunoaffinity column and undepleted plasma samples (n = 3). Three exogenous glycan standards were spiked into all samples which allowed for normalization of the N-glycan abundances. The abundances of 20 glycans varying in type, structure, composition, and molecular weight (1,200-3,700 Da) were compared between the two sets of samples. Small fucosylated non-sialylated complex glycans were found to decrease in abundance in the depleted samples (greater than or equal to tenfold) relative to the undepleted samples. Protein depletion was found to marginally effect (less than threefold) the abundance of high mannose, hybrid, and large highly sialylated complex species. The significance of these findings in terms of future biomarker discovery experiments via global glycan profiling is discussed.
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