4.5 Article

The multifunctional human p100 protein 'hooks' methylated ligands

Journal

NATURE STRUCTURAL & MOLECULAR BIOLOGY
Volume 14, Issue 8, Pages 779-784

Publisher

NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb1269

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Funding

  1. NIGMS NIH HHS [1P50 GM62407] Funding Source: Medline

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The human p100 protein is a vital transcription regulator that increases gene transcription by forming a physical bridge between promoter-specific activators and the basal transcription machinery. Here we demonstrate that the tudor and SN (TSN) domain of p100 interacts with U small nuclear ribonucleoprotein ( snRNP) complexes, suggesting a role for p100 in the processing of precursor messenger RNA. We determined the crystal structure of the p100 TSN domain to delineate the molecular basis of p100's proposed functions. The interdigitated structure resembles a hook, with a hinge controlling the movement and orientation of the hook. Our studies suggest that a conserved aromatic cage hooks methyl groups of snRNPs and anchors p100 to the spliceosome. These structural insights partly explain the distinct roles of p100 in transcription and splicing.

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