Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 395, Issue 2, Pages 473-478Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-009-2980-9
Keywords
Chip; Poly(dimethylsiloxane); External UV detection; Micro-spectrophotometer; Flow injection; Microfluidics/microfabrication; UV/Vis
Funding
- National Scientific Research Fund [Hungary K75286]
- National Office for Research and Technology [Baross-2-2007-0028]
- National Science Foundation [DMR-0351848, CHE-0515363, OISE-0754138]
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The goal of this work was to increase the sensitivity of a UV-Vis spectrophotometer by decreasing the background noise and lengthening the optical path. A microphotometer has been modified to precisely select very small parts of a microfluidic channel pattern of a chip and to measure light absorbance on a magnified area of the selected part of the channel. The viability of combining a projection microscope and a spectrophotometer for external absorbance measurements on disposable PDMS chips was studied. Besides the external direct detection above a microfluidic channel, the optical pathlength was lengthened by detecting in the region of the perpendicular exit port. Increasing the cross-sectional area of the zone of irradiation improved the signal-to-noise ratio and the limits of detection (LOD).
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