Journal
FEMS MICROBIOLOGY LETTERS
Volume 273, Issue 1, Pages 70-77Publisher
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2007.00783.x
Keywords
type II diabetes; 4-hydroxyisoleucine; biotransformation; HpcH; HpaI aldolase family
Categories
Ask authors/readers for more resources
A two-step enzymatic synthesis process of 4-hydroxyisoleucine is suggested. In the first step, the aldol condensation of acetaldehyde and alpha-ketobutyrate catalyzed by specific aldolase results in the formation of 4-hydroxy-3-methyl-2-keto-pentanoate (HMKP). In the second step, amination of HMKP by the branched-chain amino acid aminotransferase leads to synthesis of 4-hydroxyisoleucine. An enzyme possessing HMKP aldolase activity (asHPAL) was purified 2500-fold from a crude extract of Arthrobacter simplex strain AKU 626. Sequencing of the asHPAL structural gene showed that the purified enzyme belongs to the HpcH/HpaI aldolase family. The 4-hydroxyisoleucine was synthesized in vitro from acetaldehyde, alpha-ketobutyrate and L-glutamate using a coupled aldolase/branched-chain amino acid aminotransferase bienzymatic reaction.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available