Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 396, Issue 1, Pages 495-501Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-009-3195-9
Keywords
Quantitative assay; Paper; Microfluidic devices; Biomarkers
Funding
- Monash University
- Department of Chemical Engineering
Ask authors/readers for more resources
This article describes the use of microfluidic paper-based analytical devices (mu PADs) to perform quantitative chemical assays with internal standards. MicroPADs are well-suited for colorimetric biochemical assays; however, errors can be introduced from the background color of the paper due to batch difference and age, and from color measurement devices. To reduce errors from these sources, a series of standard analyte solutions and the sample solution are assayed on a single device with multiple detection zones simultaneously; an analyte concentration calibration curve can thus be established from the standards. Since the mu PAD design allows the colorimetric measurements of the standards and the sample to be conducted simultaneously and under the same condition, errors from the above sources can be minimized. The analytical approach reported in this work shows that mu PADs can perform quantitative chemical analysis at very low cost.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available