Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 393, Issue 1, Pages 171-175Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-008-2460-7
Keywords
Next-generation sequencing; Sequencing-by-synthesis; Microarrays; Microfluidics; Sequence enrichment; gDNA Library preparation
Ask authors/readers for more resources
We report a flexible method for selective capture of sequence fragments from complex, eukaryotic genome libraries for next-generation sequencing based on hybridization to DNA microarrays. Using microfluidic array architecture and integrated hardware, the process is amenable to complete automation and does not introduce amplification steps into the standard library preparation workflow, thereby avoiding bias of sequence distribution and fragment lengths. We captured a discontiguous human genomic target region of 185 kb using a tiling design with 50mer probes. Analysis by high-throughput sequencing using an Illumina/Solexa 1G Genome Analyzer revealed 2150-fold enrichment with mean per base coverage between 4.6 and 107.5-fold for the individual target regions. This method represents a flexible and cost-effective approach for large-scale resequencing of complex genomes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available