Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 391, Issue 4, Pages 1275-1281Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-007-1798-6
Keywords
tyrosinase; amperometric biosensor; phenols; Plackett-Burman design
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For detection of phenolic compounds in environmental water samples we propose an amperometric biosensor based on tyrosinase immobilized in titania sol gel. The analytical characteristics toward catechol, p-cresol, phenol, p-chlorophenol, and p-methylcatechol were determined. The linear range for catechol determination was 2.2 x 10(-7) - 1.3 x 10(-5) mol L-1 with a limit of detection of 9 x 10(-8) mol L-1 and sensitivity 2.0 x 10(3) mA mol(-1) L. The influence of sample matrix components on the electrode response was studied according to Plackett-Burman experimental design. The potential interferents Mg2+, Ca2+, HCO3-, SO42- , and Cl-, which are usually encountered in waters, were taken into account in the examination. Cu2+ was also taken into account, because CuSO4 is sometimes added to a water sample, as a preservative, before determination of phenolic compounds. It was found that among the ions tested only Mg2+ and Ca2+ did not directly affect the electrode response. The developed biosensor was used for determination of catechol in spring and surface water samples using the standard addition method.
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