Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 390, Issue 7, Pages 1829-1835Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-008-1892-4
Keywords
polycystic kidney disease (PKD); DNA; single nucleotide polymorphisms (SNPs); nanoparticles; hybridization-in-solution; lanthanide oxide
Funding
- NIEHS NIH HHS [5 P42 ES004699] Funding Source: Medline
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Rapid and accurate detection of genetic mutations based on nanotechnology would provide substantial advances in detection of polycystic kidney disease (PKD), a disease whose current methods of detection are cumbersome due to the large size and duplication of the mutated gene. In this study, a nanotechnology-based DNA assay was developed for detection of SNPs (single nucleotide polymorphisms) in a feline autosomal dominant PKD (ADPKD) model which can readily be adapted to diagnosis of human ADPKD type 1. Europium and terbium phosphors were doped into gadolinium crystal hosts with a magnetic core, providing stable luminescence and the possibility of magnetic manipulations in a solution-based assay. A hybridization-in-solution DNA assay was optimized for feline PKD gene SNP detection using genomic DNA extracted from feline kidney tissue and blood. This assay showed a substantial differentiation between PKD and control specimens. The nanotechnology-based DNA assay is attractive from the viewpoint of rapid availability, simple methodology, and cost reduction for clinical use to detect mutations involved in human ADPKD and other genetic diseases.
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