Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 391, Issue 5, Pages 1845-1852Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-008-2093-x
Keywords
optical immunosensor; river analyser (RIANA); C-reactive protein (CRP); high-sensitivity CRP (hsCRP); healthcare; TIRF
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Immunoassays are a proven approach towards fast, sensitive, cost-effective and easy-to-use analytical systems which are able to measure a variety of interesting analytes, especially in medical diagnostics. Herein, we report two assay formats, binding inhibition and sandwich assay format, for detection of C-reactive protein (CRP) in human serum. Both assays were characterised and compared with respect to their suitability and adaption into a complete sensor system. An automated, optical biosensor system, based on evanescent field technology, was used to carry out a full threefold calibration in each case. Owing to the resulting working ranges, 0.044-2.9 mg L-1 and 0.13-22.9 mg L-1, respectively, the assays qualify for use in detecting high-sensitivity CRP (C-reactive protein).
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