Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 393, Issue 6-7, Pages 1677-1684Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-008-2560-4
Keywords
NADH; Sensor; Catalytic growth; Au nanoparticles; Glassy carbon electrode
Funding
- National Natural Science Foundation of China [50608029]
- National 863 High Technology Research Program of China [2004AA649370, 2006AA06Z407]
- Chinese National Basic Research Program [2005CB724203]
- Natural Foundation for Distinguished Young Scholars [50425927, 50225926]
- Changjiang Scholars and Innovative Research Team in University [IRT0719]
- Hunan Provincial Natural Science Foundation of China [06JJ20062]
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In this work, an electrochemical dihydronicotinamide adenine dinucleotide (NADH) sensor based on the catalytic growth of Au nanoparticles (Au NPs) on glassy carbon electrode was developed. Catalyzed by Au NPs immobilized on pretreated glassy carbon electrode, the reduction of AuCl4- in the presence of hydroquinone and cetyltrimethyl ammonium chloride led to the formation of enlarged Au NPs on the electrode surface. Spectrophotometry and high-resolution scanning electronic microscope (SEM) analysis of the sensor morphologies before and after biocatalytic reaction revealed a diameter growth of the nanoparticles. The catalytic growth of Au NPs on electrode surface remarkably facilitated the electron transfer and improved the performance of the sensor. Under optimal conditions, NADH could be detected in the range from 1.25 x 10(-6) to 3.08 x 10(-4) M, and the detection limit was 2.5 x 10(-7) M. The advantages of the proposed sensor, such as high precision and sensitivity, fast response, low cost, and good storage stability, made it suitable for on-line detection of NADH in complex biological systems and contaminant degradation processes.
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