Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 390, Issue 8, Pages 2081-2088Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-008-1949-4
Keywords
molecular imprinting; synthetic receptors; endocrine disruptors; bioassay; screening; beta-estradiol
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A beta-estradiol receptor binding mimic was synthesised using molecular imprinting. Bulk polymers and spherical polymer nanoparticles based on methacrylic acid and ethylene glycol dimethacrylate as the functional monomer and crosslinker, respectively, were prepared in acetonitrile. The selectivity was evaluated by radioligand binding assays. The imprinted polymers were very specific to beta-estradiol since the control polymers bound virtually none of the radioligand. The bulk polymer was then employed to screen endocrine disrupting chemicals. Structurally related steroids like alpha-estradiol, estrone and ethynylestradiol showed, respectively, 14.0, 5.0 and 0.7% of relative binding to the beta-estradiol polymer, whereas most unrelated chemicals did not bind at all. These results are compared to those obtained with a bioassay using stably transfected yeast cells in culture bearing the human estrogen receptor. The receptor was activated by several estrogen-like chemicals and to a lesser extent by some structurally related chemicals.
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