Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 391, Issue 8, Pages 2703-2709Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-008-1994-z
Keywords
ion channel biosensor; SOI; extracellular matrix; fibronectin; nystatin
Ask authors/readers for more resources
A new planar-type ion channel biosensor with the function of cell culture has been fabricated using silicon on an insulator substrate as the sensor chip material. Coating of the sensor chip with fibronectin was essentially important for cell incubation on the chip. Although the seal resistance was quite low (similar to 7 M Omega) compared with the pipette patch-clamp gigaohm seal, the whole-cell channel current of the transient receptor potential vanilloid type 1 (TRPV1) channel expressing HEK293 cells was successfully observed, with a good signal-to-noise ratio, using capsaicin as a ligand molecule.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available