Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 73, Issue 15, Pages 5048-5051Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02973-06
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We developed a self-formed adaptor PCR (termed SEFA PCR) which can be used for chromosome walking. Most of the amplified flanking sequences were longer than 2.0 kb, and some were as long as 6.0 kb. SEFA PCR is simple and efficient and should have broad applications in the isolation of unknown sequences in complex genomes.
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