Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 393, Issue 6-7, Pages 1579-1585Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-008-2480-3
Keywords
Reflectometric interference spectroscopy (RIfS); Endocrine disruptor (EDC); Estrogen receptor (ER); Biosensor; Small interacting peptides; Label free
Funding
- European Union [FOOD-CT-2004-506319]
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A label-free and time-resolved biosensor based on reflectometric interference spectroscopy (RIfS) has been developed to evaluate the agonistic or antagonistic effects of potential ligands with unknown behavior. The biosensor utilizes the specific interaction between the estrogen receptor a (ER alpha) and short specific peptides. The unique feature of these peptides allows the investigation of the behavior of ligands and the discrimination between the agonistic and antagonistic effects caused by conformational changes of the receptor. Thus, this developed biosensor allows not only the differentiation between ligands and nonligands of a receptor, but also the potential of these ligands to influence conformational changes in the receptor, leading to activation or inhibition of the receptor-dependent pathways. Owing to the robustness of the direct optical detection principle used, the biosensor is applicable to complex biological matrices, even crude cell extracts. Moreover, the reliability of the biosensor, including regeneration steps when performing subsequent measurements, has been verified.
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