Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 392, Issue 7-8, Pages 1291-1297Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-008-2433-x
Keywords
Liquid chromatography; Shotgun proteomics; Monoliths; Nano-LC columns; Peak capacity
Funding
- Marie Curie reintegration [MIRG-CT-2006-030810]
- International Association INTAS [05-1000004-7759]
- CEA (iRTSV/EDyP
- CNRS [UMR5168]
- Universite Joseph Fourier, Grenoble, France
Ask authors/readers for more resources
We report a premier side-by-side comparison of two leading types of monolithic nano-LC column (silica-C-18, polystyrene) in shotgun proteomics experiments. Besides comparing the columns in terms of the number of peptides from a real-life sample (Arabidopsis thaliana chloroplast) that they identified, we compared the monoliths in terms of peak capacity and retention behavior for standard peptides. For proteomics applications where the mobile phase composition is constrained by electrospray ionization considerations (i.e., there is a restricted choice of ion-pairing modifiers), the polystyrene nano-LC column exhibited reduced identification power. The silica monolith column was superior in all measured values and compared very favorably with traditional packed columns. Finally, we investigated the performances of the monoliths at high flow rates in an attempt to demonstrate their advantages for high-throughput identification.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available