Journal
ANALYTICA CHIMICA ACTA
Volume 1041, Issue -, Pages 102-107Publisher
ELSEVIER
DOI: 10.1016/j.aca.2018.08.052
Keywords
Antibody; Inhibition; Multiplexed detection; Stretchable DNAzyme
Categories
Funding
- National Natural Science Foundation of China [81772593, 81672570, 21235003]
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Advanced methods for developing and applying responsive DNA nanodevices are of great interest. Herein, we report a stretchable DNAzyme that allows simple, multiplexed and sensitive fluorescent detection of antibodies. We find that rigid antibody can tightly stretch soft, antigen-labelled DNAzyme strand and disrupt the hybridization between DNAzyme and its substrate. Based on this finding, we develop a novel strategy to detect antibodies. Due to the robustness and high activity of DNAzyme, this assay can easily detect target as low as 1 +/- 0.25 pM and achieve multiplexed detection by using a cocktail of DNAzymes. The proposed assay not only provides a new approach to readily measure antibody, but broadens the application of DNAzyme that is usually employed to detect metal ions or indirectly analyze biomolecules without the cumbersome design. (C) 2018 Elsevier B.V. All rights reserved.
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