Journal
ANALYTICA CHIMICA ACTA
Volume 1042, Issue -, Pages 116-124Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2018.08.038
Keywords
Gold nanoparticles; Polyamidoamine dendrimers; Assemble; Enzyme-linked immunosorbent assay; Signal amplification
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Funding
- National Natural Science Foundation of China [21502056]
- Natural Science Foundation of Guangdong Province, China [2016A030310463]
- Fundamental Research Funds for the Central Universities [2017MS079]
- Medical Scientific Research Foundation of Guangdong Province, China [A2018080]
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Amplifying the signal of ELISA is important in the early disease diagnosis. Herein we report an ultra-sensitive ELISA applying for the detection of hCG based on the assemble of AuNPs induced by functional PAMAM. The AuNP-PAMAM probe shows a competitive advantage sensitivity of 0.03 IU L-1 compared to traditional ELISA and mAb1-AuNP-HRP probe. The line range is ranged from 0.1 to 6.4 IU L-1. Moreover, the precision and reproducibility and specificity of AuNP-PAMAM probe are also eligible for the detection of hCG. The assembled AuNPs was firstly used in the signal enhancement in immunoassay. (C) 2018 Elsevier B.V. All rights reserved.
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