Journal
ANALYTICA CHIMICA ACTA
Volume 812, Issue -, Pages 145-151Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2013.12.024
Keywords
Fluorescent probes; Fluorescence; Dyes/pigments; Ratiometric; Iron
Categories
Funding
- National Natural Science Foundation of China [21202063, 50925207, 51172100, 21103073]
- Ministry of Science and Technology of China [2009DFA50620, 2011DFG52970]
- Ministry of Education of China [IRT1064]
- Natural Science Foundation of Jiangsu Province [BK2012281]
- China Postdoctoral Science Foundation [2012M511200]
- Jiangsu Innovation Research Team, and the Research Foundation ofJiangsu University [11JDG078]
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A simple and versatile ratiometric fluorescent Fe3+ detecting system, probe 1, was rationally developed based on the Fe3+-mediated deprotection of acetal reaction. Notably, this reaction was firstly employed to design fluorescent Fe3+ probe. Upon treatment with Fe3+, probe 1 showed ratiometric response, with the fluorescence spectra displaying significant red shift (up to 132 nm) and the emission ratio value (I522/I390) exhibiting approximately 2362-fold enhancement. In addition, the probe is highly sensitive (with the detection limit of 0.12 mu M) and highly selective to Fe3+ over other biologically relevant metal ions. The sensing reaction product of the probe with Fe3+ was confirmed by NMR spectra and mass spectrometry. TD-DFT calculation has demonstrated that the ratiometric response of probe 1 to Fe3+ is due to the regulation of intramolecular charge transfer (ICT) efficiency. Moreover, the practical utility in fluorescence detection of Fe3+ in human blood serum was also conducted, and probe 1 represents the first ratiometric fluorescent probe that can be used to monitor Fe3+ level in human blood serum. Finally, probe 1 was further employed in living cell imaging with pancreatic cancer cells, in which it displayed low cytotoxicity, satisfactory cell permeability, and selective ratiometric response to Fe3+. (C) 2013 Elsevier B.V. All rights reserved.
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