Journal
ANALYTICA CHIMICA ACTA
Volume 812, Issue -, Pages 236-242Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2014.01.021
Keywords
Chemiluminescence; Luminol functionalized silver nanoprobe; Chloramphenicol; Immunoassay
Categories
Funding
- National Natural Science Foundation of PR China [21173201, 21075115, 20625517, 20573101]
- Fundamental Research Funds for the Central Universities [WK2060190007]
- Overseas Outstanding Young Scientist Program of the Chinese Academy of Sciences
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Chloramphenicol (CHL) as a broad-spectrum antibiotic has a broad action spectrum against Gram-positive and Gram-negative bacteria, as well as anaerobes. The use of CHL is strictly restricted in poultry because of its toxic effect. However, CHL is still illegally used in animal farming because of its accessibility and low cost. Therefore, sensitive methods are highly desired for the determination of CHL in foodstuffs. The immunoassays based on labeling as an important tool have been reported for the detection of CHL residues in food-producing animals. However, most of the labeling procedures require multi-step reactions and purifications and thus they are complicated and time-consuming. Recently, in our previous work, luminol functionalized silver nanoparticles have been successfully synthesized, which exhibits higher CL efficiency than luminol functionalized gold nanoparticles. In this work, the new luminol functionalized silver nanoparticles have been used for the labeling of small molecules CHL for the first time and a competitive chemiluminescent immunoassay has been developed for the detection of CHL. Owing to the amplification of silver nanoparticles, high sensitivity for CHL could be achieved with a low detection limit of 7.6 x 10(-9) g mL(-1) and a wide linear dynamic range of 1.0 x 10(-8)-1.0 x 10(-6) g mL(-1). This method has also been successfully applied to determine CHL in milk and honey samples with a good recoveries (92% and 102%, 99% and 107% respectively), indicating that the method is feasible for the determination of CHL in real milk and honey samples. The labeling procedure is simple, convenient and fast, superior to previously reported labeling procedures. The immunoassay is also simple, fast, sensitive and selective. It is of application potential for the determination of CHL in foodstuffs. (C) 2014 Elsevier B.V. All rights reserved.
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