Journal
ANALYTICA CHIMICA ACTA
Volume 820, Issue -, Pages 152-158Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2014.02.043
Keywords
Immunoassay; Enrofloxacin; Heterologous hapten; Cross-reactivity; Chicken muscle
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Funding
- Natural Science Foundation of China [U1301214, 31372475]
- Trans-Century Training Programme Foundation for the Talents by the Ministry of Education [NCET-12-0529]
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In the paper, an enzyme-linked immunosorbent immunoassay (ELISA) for detection of enrofloxacin was described using one new derivative of enrofloxacin as coating hapten, resulting in surprisingly high sensitivity and specificity. Incorporation of aminobutyric acid (M) in the new derivative of enrofloxacin had decreased the IC50 of the ELISA for enrofloxacin from 1.3 mu g L-1 to as low as 0.07 mu g L-1. The assay showed neglect cross-reactivity for other fluoroquinolones but ofloxacin (8.23%), marbofloxacin (8.97%) and pefloxacin (7.29%). Analysis of enrofloxacin fortified chicken muscle showed average recoveries from 81 to 115%. The high sensitivity and specificity of the assay makes it a suitable screening method for the determination of low levels of enrofloxacin in chicken muscle without clean-up step. (C) 2014 Elsevier B.V. All rights reserved.
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