Journal
ANALYTICA CHIMICA ACTA
Volume 841, Issue -, Pages 99-105Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2014.05.037
Keywords
Glycopeptides; Magnetic colloidal nanocrystal clusters; Chitosan; Hydrophilic interaction liquid; chromatography
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Funding
- China State Key Basic Research Program [2013CB911202]
- Creative Research Group Project of NSFC [21321064]
- National Natural Sciences Foundation of China [21075105, 21165017]
- Educational Commission of the Xinjiang Uygur Autonomous Region of China [XJEDU2010I03]
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Selective enrichment of glycopeptides prior to the mass spectrometry (MS) analysis is essential due to ion suppression effect during ionization caused by the co-presence of non-glycosylated peptides. Among the enrichment approaches, hydrophilic interaction liquid chromatography (HILIC) based on magnetic separation has become a popular method in recent years. As the conventional synthesis procedures of these materials are tedious and time-consuming with at least four steps. Herein, magnetic colloidal nanocrystal clusters coated with chitosan (Fe3O4@CS MCNCs) have been successfully prepared by a simple one-pot method. The resulting Fe3O4@CS MCNCs demonstrated an excellent ability for glycopeptide enrichment with high selectivity, low detection limit and high binding capacity. Furthermore, in the analysis of real complicated biological sample, 283 unique N-glycosylation sites corresponding to 175 glycosylated proteins were identified in three replicate analyses of 45 mu g protein sample extracted from HeLa cells, indicating the great potential in detection and identification of low abundant glycopeptides in glycoproteome analysis. (C) 2014 Elsevier B.V. All rights reserved.
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