Journal
ANALYTICA CHIMICA ACTA
Volume 844, Issue -, Pages 35-43Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2014.07.032
Keywords
Perfluorinated compounds (PFCs); Fluorous solid phase extraction (F-SPE); Magnetic mesoporous microspheres; High-performance liquid chromatography coupled to tandem mass spectrometry?(LC-MS/MS); Human serum
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Funding
- National Natural Science Foundation of China [21105016]
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We report herein an extraction method for the analysis of perfluorinated compounds in human serum based on magnetic core-mesoporous shell microspheres with decyl-perfluorinated interior pore-walls (Fe3O4@mSiO(2)-F-17). Thanks to the unique properties of the Fe3O4@mSiO(2)-F-17 microspheres, macromolecules like proteins could be easily excluded from the mesoporous channels due to size exclusion effect, and perfluorinated compounds (PFCs) in protein-rich biosamples such as serum could thus be directly extracted with the fluorocarbon modified on the channel wall without any other pretreatment procedure. The PFCs adsorbed Fe3O4@mSiO(2)-F-17 microspheres could then be simply and rapidly isolated by using a magnet, followed by being identified and quantified by LC-MS/MS (high-performance liquid chromatography coupled to tandem mass spectrometry). Five perfluorinatedcarboxylic acids (C6, C8-C11) and perfluorooctane sulfonate (PFOS) were selected as model analytes. In order to achieve the best extraction efficiency, some important factors including the amount of Fe3O4@mSiO(2)-F-17 microspheres added, adsorption time, type of elution solvent, eluting solvent volume and elution time were investigated. The ranges of the LOD were 0.02-0.05 ng mL (1) for the six PFCs. The recovery of the optimized method varies from 83.13% to 92.42% for human serum samples. (C) 2014 Elsevier B.V. All rights reserved.
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