4.7 Article

Carbon dots from tryptophan doped glucose for peroxynitrite sensing

Journal

ANALYTICA CHIMICA ACTA
Volume 852, Issue -, Pages 174-180

Publisher

ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2014.08.050

Keywords

Carbon dots; Tryptophan; Peroxynitrite; Sensing; Fluorescence quenching

Funding

  1. Fundacao para a Ciencia e Tecnologia (FCT, Lisbon) [SFRH/BD/81074/2011]
  2. Fundação para a Ciência e a Tecnologia [SFRH/BD/81074/2011] Funding Source: FCT

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Tryptophan doped carbon dots (Trp-CD) were microwave synthesized. The optimum conditions of synthesizing of the Trp-CD were established by response surface multivariate optimization methodologies and were the following: 2.5 g of glucose and 300 mg of tryptophan diluted in 15 mL of water exposed for 5 min to a microwave radiation of 700 W. Trp-CD have an average size of 20 nm, were fluorescent with a quantum yield of 12.4% and the presence of peroxynitrite anion (ONOO ) provokes quenching of the fluorescence. The evaluated analytical methodology for ONOO detection shows a linear response range from 5 to 25 mu M with a limit of detection of 1.5 mu M and quantification of 4.9 mu M. The capability of the ONOO quantification was evaluated in standard solutions and in fortified serum samples. (C) 2014 Elsevier B. V. All rights reserved.

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