Journal
ANALYTICA CHIMICA ACTA
Volume 762, Issue -, Pages 83-86Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2012.11.056
Keywords
Enzyme mimics; Nanoclusters; Biosensors; Copper; Peroxidase
Categories
Funding
- National Natural Science Foundation of China [21175126]
- Faculty Development Program of Bahauddin Zakaryia University, Multan [PF/Cont./2-50/Admin/1830]
- Chinese Academy of Sciences
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Copper nanoclusters (Cu NCs) are found to possess intrinsic peroxidase-like activity for the first time. Similar to nature peroxidase, they can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine by H2O2 to produce a nice blue color reaction. Compared with horseradish peroxidase, Cu NCs exhibits higher activity near neutral pH, which is beneficial for biological applications. The increase in absorbance caused by the Cu NCs catalytic reaction allows the detection of H2O2 in the range of 10 mu M to 1 mM with a detection limit of 10 mu M. A calorimetric method for glucose detection was also developed by combining the Cu NCs catalytic reaction and the enzymatic oxidation of glucose with glucose oxidase. Taking into account the advantages of ultra-small size, good stability, and high biocompatibility in aqueous solutions, Cu NCs are expected to have potential applications in biotechnology and clinical diagnosis as enzymatic mimics. (C) 2012 Elsevier B.V. All rights reserved.
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