Journal
ANALYTICA CHIMICA ACTA
Volume 800, Issue -, Pages 71-76Publisher
ELSEVIER
DOI: 10.1016/j.aca.2013.09.018
Keywords
Hypochlorous acid; Fluorescent probe; Boron dipyrromethene; Bioimaging
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Funding
- National Science Council (ROC)
- National Chiao Tung University
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A boron dipyrromethene (BODIPY)-based fluorometric probe, HCS, has been successfully developed for the highly sensitive and selective detection of hypochlorous acid (HOCl). The probe is based on the specific HOCl-promoted oxidation of methyl phenyl sulfide. The reaction is accompanied by a 160-fold increase in the fluorescent quantum yield (from 0.003 to 0.480). The fluorescent turn-on mechanism is accomplished by suppression of photoinduced electron transfer (PET) from the methyl phenyl sulfide group to BODIPY. The fluorescence intensity of the reaction between HOCl and HCS shows a good linearity in the HOCl concentration range 1-10 mu M. The detection limit is 23.7 nM (S/N = 3). In addition, confocal fluorescence microscopy imaging using RAW264.7 macrophages demonstrates that the HCS probe could be an efficient fluorescent detector for HOCl in living cells. (C) 2013 Published by Elsevier B.V.
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