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JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 129, Issue 31, Pages 9564-+Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja072884t
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Analysis of the first gene cluster encoding cytochalasan biosynthesis and targeted RNA silencing in Penicillium expansum revealed that these complex tricyclic natural products are assembled by a fungal PKS-NRPS hybrid synthase, CheA. A biosynthetic Diels-Alder macrocyclization can be rationalized on the basis of the deduced enzyme functions.
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