Journal
EMBO JOURNAL
Volume 26, Issue 15, Pages 3699-3708Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.emboj.7601790
Keywords
cancer; genetic screen; miRNA; p27
Categories
Ask authors/readers for more resources
MicroRNAs (miRNAs) are potent post-transcriptional regulators of protein coding genes. Patterns of misexpression of miRNAs in cancer suggest key functions of miRNAs in tumorigenesis. However, current bioinformatics tools do not entirely support the identification and characterization of the mode of action of such miRNAs. Here, we used a novel functional genetic approach and identified miR-221 and miR-222 (miR-221& 222) as potent regulators of p27(Kip1), a cell cycle inhibitor and tumor suppressor. Using miRNA inhibitors, we demonstrate that certain cancer cell lines require high activity of miR-221& 222 to maintain low p27(Kip1) levels and continuous proliferation. Interestingly, high levels of miR-221& 222 appear in glioblastomas and correlate with low levels of p27(Kip1) protein. Thus, deregulated expression of miR-221& 222 promotes cancerous growth by inhibiting the expression of p27(Kip1).
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available